Mitochrome, a New Respiratory Enzyme. I. The Mediation of Activated Phosphate Transformations in Mitochondria

摘要

A new chromoprotein, mitochrome, released from mito¬chondria during ageing or other damaging processes, has been isolated in pure form. Physical-chemical determinations of partial specific volume, diffusion constant, and sedimentation constant permitted the calculation of a molecular weight in the order of 100, 000 in agreement with the value calculated from the iron content of 0.055 percent, assuming one atom of iron per molecule of mitochrome.nElectrophoretic studies indicated a single component protein both on the acid and alkaline side of the isoelectric point at pH 5.1. Spectrophotometric analysis revealed two bands at 278 and 419 mμ. Reduction with dithionite displaced the Soret band to 422 mμ.nElectrophoretic and sedimentation experiments furnish evidence for a protein-protein interaction between mitochrome and serum albumin.