'In vivo' and 'In vitro' Multinuclear Magnetic Resonance Studies of the Energy Metabolism of Methanogens. Final Report April 1984-April 1986

摘要

Methanogens are fastidious anaerobes whose metabolism centers around the reduction of CO2 to CH4, because of their unique biological niche, methanogens possess biosynthetic and energy metabolism pathways that differ from other organisms that fix CO2. The authors have used in vivo and in vitro multinuclear magnetic resonance spectroscopy to monitor energy metabolism, carbon assimilation, and nitrogen flow in Methanobacterium thermoautotrophicum. Specific results include (1) elucidation of a biosynthetic role for 2,3-cyclopyrophosphoglyceric acid, CPP (the major carbon and phosphorus pool in Mb. thermoautotrophicum), in carbohydrate metabolism, (2) evidence for strict metabolic control of CPP biosynthesis and degradation, (3) evidence via analysis of 13C-13C coupling patterns in cyclopyrophosphoglycerate for the asymmetric condensation of two CO2 molecules to an acetate unit and examination of how several metabolic poisons affect the condensation in vivo.