Developmental Approach to the Study of Immunologic Tolerance. Final Report

摘要

The effect of ethyl stearate and methyl palmitate on the reticuloendothelial systems (RES) of BALB/c and C57B1/6 mice has been studied. The ease of induction of immunologic tolerance to gamma globulin from different mammalian species has been studied. Several compounds have been evaluated as potential probes to ascertain the intracellular events which transpire during the induction of immune tolerance. As the cyclic nucleotides have been implicated as moderators of immune responses, it was of interest to determine the effect, if any, that agents which are known to modulate the intracellular concentration of these compounds may have on the induction of tolerance. Mitomycin C, an inhibitor of DNA replication, was studied as a possible probe to ascertain the need for cell division during tolerance induction. Actinomycin D, an inhibitor of DNA directed RNA synthesis, was next evaluated for its ability to interfere with tolerance induction. A transfer system was used to explore the metabolic requirements for tolerance induction to human gamma globulin in the murine thymocyte. (ERA citation 05:021281)