Diagnose und Therapie von Funktionsstoerungen menschlicher Leukozten nach Bestrahlung (Diagnosis and Therapy of Dysfunctions of Human Leukocytes After Irradiation)

摘要

Infections are an important aspect of the hematopoietic syndrome of acute radiation sickness. Pathophysiological infections arc caused by depletions and/or functional deficiencies of white blood cells as major carriers of cellular immunity. Polymorphonuclear neutrophilic granulocytes (PMN) are important as the first immunological barrier. Experiments were performed with isolated human PMN and with the promyelecytic HL-60 cell line, induced to differentiate along the granulocytic lineage with dimethyl sulfoxid. The respiratory burst reaction was triggered with soluble stimuli and measured flow cytometrically or a chemiluminescence signal with indicator molecules dihydrorhodamine 123 or luminol. The presence of enzymes was postulated, verified with Western blotting, and their activities were inhibited pharmacologically. Lipid 2nd messengers were measured by high-performance liquid chromatography.