Characterization of Two Proteins Which Interact With the BRCA 1 Gene.

摘要

We have made extensive progress to support our analysis of the BAPl- BRCAl interaction. We have published the co-localization and cell-cycle patterns of intranuclear distribution for the two proteins. In addition, we have shown that BAP-1 is a bonafide tumor suppressor gene which is able to inhibit the malignant growth characteristics of cell lines when transfected back into the BAP1 null cells. This activity is dependent upon enzymatic function of the ubiquitin hydrolysis domain. Our recent studies are aimed at exploring whether BAP1, like BRCAl, might have a direct role in transcription-coupled DNA repair. The work accomplished conforms to the original SOW and has shed new light on the role of BRCAl as a tumor suppressor in early onset familial breast-ovarian cancer families.