Proanthocyanidin-based Endotoxin Removal.

摘要

The application of proanthocyanidins (PACs) to the capture and removal of bacterial lipopolysaccharide (LPS) from solution has been demonstrated. PACs from whole cranberries, grape juice, black tea, and cranberry juice were purified and immobilized onto thiol-activated Sepharose beads. This material was used in pull-down type assays for the capture of LPS. The binding of LPS by PACs has been shown to compete with that of polymyxin B, which is known to bind the lipid A component of LPS. The removal of LPS from solution using a column of a PAC modified resin is also demonstrated over a range of pH values and ionic strengths. The performance of the resin in protein solutions as compared to that of polymyxin B is evaluated. PACs were also immobilized onto Luminex beads and applied to the capture of E. coli and B. globigii.