One Carbon Metabolism and Methylation in Breast Tumors

摘要

The first aim of this case control study is to examine the risk of breast cancer in relation to germ-line variants found in three key enzymes related to one-carbon metabolism. We also plan to determine the Methylation status of several genes in our cases and to identify any association with polymorphisms found in enzymes related to one-carbon metabolism. Finally we determined the ER status of all cases in an attempt to identify associations with p53 mutations. In the last year I have completed genotyping on all cases and controls for polymorphisms in 4 genes, determined the Methylation status of 4 genes for a fraction of all cases, and completed ER staining and scoring on all cases. MTHFR C677T data has been analyzed and the results indicated an increase breast cancer risk for the TT variant. This increased risk was further influenced by a high alcohol and low folate intake especially in our postmenopausal women. The analysis other SNF data has not been completed.