Novel in Vitro System for Exposures of Cell Cultures to Volatile Chemicals

摘要

The potential toxic effects of chromophore powder in primary hepatocytes were investigated. Initially, a solubility assessment was conducted in aqueous and organic solutions. The solubility test showed that the chromophore powder is soluble in DMSO at 0.001 mg/ml. while not in water or ethyl alcohol. Therefore, DMSO was selected as the solvent for the dose range funding study. For initial toxicity evaluations mitochondrial function (MTT assay). lactate dehydrogenase release (LDH assay) and the morphology of cells were assessed for primary hepatocytes under control or exposed conditions (2 hour treatment followed by 24 h of incubation in fresh media). The microscopic studies demonstrated that chromophore-exposed cells at the doses of 0.5 and 1 mg/ml became abnormal in size displaying shrinkage and irregular shape. The results showed that chromophore powder had no measurable effect on mitochondrial function. However, LDH assay showed a significant increase in LDH release at O. 1mg/ml. while there was no change at 0.5 and 1.0 mg/ml. The dose range funding study indicates that chromophore powder displayed limited toxicity in vitro at increasing dose.