Instant Cell Analysis, BioSPICE, Cellular Devices and Exquisite Detection: Towards an Interactive Biology

摘要

The overall objective of this project was to rapidly find molecular reagents to interfere or bind to every gene product in the Saccharomyces cerevisiae through the development of new screening and data analytical technologies. Two experimental approaches were taken. Ron Davis developed the haploinsufficiency profiling (HIP) technology wherein two libraries of yeast were constructed one containing strains missing one copy the other strains missing both copies of every gene in yeast (that could be knock out). In place of its missing gene, each strain was engineered with a DNA bar code to identify it. Affymetrix chips were used to measure the relative populations of each strain under different chemical perturbations. Roger Brent worked on build protein aptamers to bind gene products. Arkin's role was in the development of analyses for the Haploinsufficiency data.