Development of a Novel Vector for Multiple CDC Category a Pathogens

摘要

Specific Aim 1 was to generate a panel of RhCMV/MPV vectors expressing MPV antigens A29L, A35R, M1R and B6R in either the wild type RhCMV vector, or in a vector lacking MHC immunomodulatory genes. Vectors have been constructed and characterized, and we have subsequently selected one WT RhCMV vector (WTRhCMV/A35R) for immunogical characterization in rhesus macaques. Specific Aims 2 and 3 were to establish the pathobiology of WT MPV infection in RMs, and to monitor the immunological consequences of WT MPV infection. To date, six RMs have been experimentally inoculated intrabronchially with MPV Zaire strain. Two with 2 x 107 plaque forming units (PFU) and four with 2 x 105 PFU, to define a lethal dose by this route of infection and to characterize the virus/host interactions.