Production of Murine Monoclonal Antibodies using Traditional and Novel Technology

摘要

Recent attempts to generate monoclonal antibodies against various biological immunogens have proven to be successful. This report discusses the traditional and novel technologies used to produce monoclonal antibodies. Hybridoma monoclonal antibodies are highly specific antibodies produced in large quantities by cloning a single hybrid cell formed by the fusion of a murine B cell and a tumor cell. Recent attempts to generate monoclonal antibody against Ricin B-Chain and Ovalbumin have proven to be successful. After a pre- determined immunization period, the murine spleens containing the B cells are harvested and fused together with a tumor cell (SP2/0) to generate the first round of monoclonal cells. Subsequent screenings are conducted using the enzyme linked immuno sorbent assay (ELISA) followed by additional cloning steps. The resulting cell lines are stable, monoclonal hybridomas. The supernatant generated from the cell lines are protein purified and tested via ELISA for cross-reactivity. The final product is a hybridoma monoclonal antibody specific to the target for which it was originally immunized.