Enzyme Activity in the Serum and Common Duct Bile of Dogs.

摘要

The observations that patients with obstructive jaundice show elevated levels of serum glutamic oxalacetic transaminase and that ligation of the common bile duct in rats results in similar enzyme changes prompted direct investigation of the excretory pathway of the serum enzyme protein moieties. Mongrel dogs, subjected to cholecystectomy and ligation of the common bile duct, were used. Exposure of these animals to carbon tetrachloride vapor, with the expected massive increase in serum glutamic oxalacetic transaminase, failed to result in increased bile levels of the enzyme. Correspondingly, when pure enzyme was administered intravenously to these animals increased serum activity levels failed to induce a corresponding elevated bile activity. These data indicate that if the bile serves as an excretory pathway for serum enzymes, such pathway is minor.