Fluorescent Titrations at Low Protein Concentrations.

摘要

Analyses of errors resulting from the uncritical use of the 1/F versus 1/L plot to obtain the dissociation constand, K sub d, of a ligand-protein complex are shown. Similarly, the 'two titration' method for obtaining the association constant, K sub a, and the number of moles of ligand bound/mole of protein is error analyzed. Strategies for optimum utilization of the two techniques are presented. The latter technique is suggested to be the most helpful and amenable to internal analysis. (Author)