Immunologic Cross-Reactivity Between Animal Tissue and Enterobacterial Common Antigen (CA).

摘要

The purpose of the current study was to (1) assess the immunogenicity of enterobacterial common antigen (CA) in the guinea pig as measured by cutaneous delayed by hypersensitivity and humoral antibody response to CA; (2) determine whether protection against infectious challenge, as measured by survival, is evoked in mice immunized with erythrocytes treated with CA; (3) assess strains of out-bved, in-bved and gnotobiotic mice for a tissue antigen cross-reactive with CA as measured by the capacity of tissue extracts to modify RBC for hemagglutination and to inhibit CA hemagglutination. CA engendered both humoral and cellular responses in guinea pigs. Swiss white albino mice immunized with heterologous erythrocytes treated with CA are significantly, albeit transiently, protected against infectious challenge with a CA producing enteropathogen. An antigen which cross-reacts with CA was demonstrated in the spleens, livers, or kidneys of Swiss white albino, CS7BL/6HA, and DBA mice. Likely, the reactions observed by hemagglutination-inhibition and rabbit-immunization protocols do not reflect contamination by enteric microbial flora; tissues excised from gnotobiotic Swiss white albino mice produced similar patterns of cross-reactivity with CA.