Trypanosoma b. Rhodesiense (WRATat Serodeme): Purfication and Characterization of Surface Antigens for the Vaccine Development Program

摘要

The variant-specific glycoproteins (VSG's) from some of the antigenic clones of organisms of the Trypanosoma b. rhodesiense WRATat serodeme were purified from cell lysates. Pooled eluate-fractions of lectin-affinity chromatography using concanavalin-A agarose was the primary fractionation tool followed by gel permeation chromatography on TSK, SW-3000 HPLC columns. VSG's which showed single band purity by denaturing polyacrylamide gel electrophoresis were hydrolized with 3N mercaptoethanesulfonic acid and their amino acid compositions determined. Studies were initiated to determine how to manipulate the rats and the parasites to obtain better and consistent yields of trypanosomes from the blood of the infected animals. Host responses to acute infections were measured for changes in glucose, insulin, multiplication-stimulating activity (MSA), nonsuppressible insulin-like activity (NSILA), lactate, catecholamines and others. Attempts were made to isolate and clone acute, isoantigenic trypanosomes from the terminal waves of parasitemia initiated with one of the chronic clones of T.b. rhodesiense WRATat-5. An acute strain was isolated, cloned and designated T.b. rhodesiense CSUT-J1.