Evaluating Interstrand Crosslink (ICL) Repair in Triple-Negative Breast Cancers to Guide Chemotherapy.

摘要

The main goals of this proposal are: 1) to develop and optimize a rapid method to assess the ability of breast tumor cell lines to repair specifically DNA interstrand crosslinks (ICLs). 2) to apply this technology to a set of human breast cancer cell lines. In addition, we propose to compare ICLs repair with surrogate markers for ICL repair. The predicted outcome of these studies is to identify a subset of human breast tumor cell lines that are cisplatinsensitive and to correlate this sensitivity with their ability to repair ICLs. We hypothesized that breast cancer cell lines with defects in ICL repair will fall into the basal-like/triple-negative breast cancer category of tumor cell lines and correlate with an increased sensitivity to cisplatin.