Effect of Root-Surface Modification of Human Teeth on Adherence of Fibronectin.

摘要

Fibronectin (FN) is a promising agent in periodontal disease therapy for facilitating reattachment of connective tissue to human tooth-root surfaces. This study examined the influence of various surface treatments on promoting or hindering FN adherence to tooth roots. Rectangular sections of planed and non-planed tooth-root surfaces were acid-demineralized or incubated with collagenase. The tooth-root sections were treated with tritiated (FN) and its adherence was determined by liquid scintillation counting. The level of FN adherence was highest for non-planed acid-demineralized sections, and lowest for planed-only sections. This adherence varied with FN concentration and duration of contact for non-planed sections, but varied only with duration of contact for planed sections. Collagenase significantly reduced FN adherence to non-planed or planed acid-demineralized sections, but not to planed-only sections. These effects were attributed to tooth-root collagen, which can strongly bind FN. Keywords: Fibronectin; Root-surface; Root-planing; Demineralization; Collagen; Collagenase. (kt)