Chemoprevention of Prostate Cancer Initiation in a Novel Transgenic Mouse Model by Targeting 15-Lipoxygenase-1.

摘要

FLiMP mice, which conditionally express prostatic human 15-LO-1, display mouse prostatic intraepithelial neoplasia (mPIN) by week 20, but do not progress to cancer when on normal diet. To examine for the up-regulated and downregulated genes in the prostates of FLiMP we used pooled cDNA sets of individual prostate regions from FLiMP+/- and FLiMP+/+ mice and age matched nontransgenic C57BL/6 littermates were used for cDNA production. Hybridization was performed on pretreated 38.5K mouse Illumina MEEBO oligonucleotide (25,000 genes) microarray slides. The slides were scanned and intensity data were further analyzed with GeneSpring 7.0 software and normalized. All of the data were filtered very heavily yielding a total of 7037 genes. This list was used as the starting list for fold-change calculations.