Mutagenic Potential of 2-Hydroxyiminomethyl-3-methyl-1-(1-(2-methyl-2- nitropropyloxymethyl))imidazolium Chloride (WR 254,933) in the Ames Salmonella/Mammalian Microsome Mutagenicity Test

摘要

2-HYDROXYIMINOMETHYL-3-METHYL-1-(1-(2-METHYL-2-NITROPROPYLOXYMETHYL)) IMIDAZOLIUM CHLORIDE (WR 254,933) was synthesized for a United States Army Medical Research and Development Command program charged with developing more effective oximes for treatment of nerve agent poisoning. The Ames Salmonella/Mammalian Microsome Mutagenicity Test is one of a series of tests in which these compounds will be evaluated to determine their relative potential for further development. The Ames Test is a short-term screening test that utilizes histidine auxotrophic mutant strains of Salmonella typhimurium to detect compounds that are potentially mutagenic in mammals. A mammalian microsomal enzyme system is incorporated in the test to increase sensitivity by simulating in vivo metabolic activation of the test compound. The Ames test is an inexpensive yet highly predictive and reliable test for detecting mutagenic activity and thus carcinogenic potential. Normal results were obtained for all sterility and strain verification tests during the Ames Test and WR254,933 did not induce any appreciable increase in the revertant colony counts relative to those of the negative control cultures. (kt)