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PROCESS FOR DETECTING MUTATIONS, TRANSGENIC MAMMAL, TRANSGENIC MAMMALIAN CELL, AND PROCESS FOR TESTING AGENTS OR CONDITIONS FOR MUTAGENIC PROPERTIES
PROCESS FOR DETECTING MUTATIONS, TRANSGENIC MAMMAL, TRANSGENIC MAMMALIAN CELL, AND PROCESS FOR TESTING AGENTS OR CONDITIONS FOR MUTAGENIC PROPERTIES
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机译:检测突变,转基因哺乳动物,转基因哺乳动物细胞的过程以及检测突变特性的试剂或条件的过程
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摘要
Detection of mutations das a lacZ gene including lacZ operator sequence a serving marker gene located in a plasmid which was introduced one or more copies in the DNA of a mammal or a mammalian cell. This detection method comprises isolating DNA, cut plasmid DNA, recovering the plasmid DNA using solid particles to which is bound an operator binding material lacZ, to release the new particle plasmid after isolation then circularized to transform using the plasmid a bacterial host restriction-negative host, lacZ-negative and galE-negative, and separating the transformants with a mutant lacZ gene growing on a medium containing lactose, transformants having a non-mutant lacZ gene and which do not grow on such a medium. Is also provided a transgenic mammal model for mutation analysis.
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