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PROCESS FOR DETECTING MUTATIONS, TRANSGENIC MAMMAL, TRANSGENIC MAMMALIAN CELL, AND PROCESS FOR TESTING AGENTS OR CONDITIONS FOR MUTAGENIC PROPERTIES
PROCESS FOR DETECTING MUTATIONS, TRANSGENIC MAMMAL, TRANSGENIC MAMMALIAN CELL, AND PROCESS FOR TESTING AGENTS OR CONDITIONS FOR MUTAGENIC PROPERTIES
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机译:检测突变,转基因哺乳动物,转基因哺乳动物细胞的过程以及检测突变特性的试剂或条件的过程
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摘要
Detection of mutations in a lacZ gene including lacZ operator sequence functioning as marker gene located in a plasmid whereof one or more copies are introduced into the DNA of a mammal or of a mammalian cell, by isolating the DNA, cutting the plasmid out of the DNA, picking up the plasmid DNA with solid particles to which a lacZ operator binding material is bound, again releasing the plasmid from the solid particles after isolation and subsequently circularizing the plasmid, transforming a host restriction-negative, lacZ-negative and galE-negative bacterial host with the plasmid and separating transformants with mutated lacZ gene which grow on a lactose-containing medium from transformants with a non-mutated lacZ gene which do not grow on such a medium. A transgenic mammal model for mutation analysis.
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