Effect of Hyperbaric Oxygen and Pentoxifylline on the Rate of Neovascularizationin Mice

摘要

A polyvinyl alcohol sponge was implanted in mouse subcutaneous tissue toinvestigate two treatments INTERMITTENT HYPEROXIA (100% OXYGEN FOR 90 MINS TWICE A DAY AT 250 KPa) and epidermal growth factor (EGF) which may modulate fibroblast infiltration. Two conditions were established for treatment: exposure of animals to chronic hypoxia (12% oxygen for 23 hr/day), simulating low oxygen tensions in problem wounds, and normoxia (21% oxygen). In experiments evaluating EGF, sponges were implanted whose core contained EGF covered with a slow release polymer, the other group with placebo. Sponges were harvested at 15, 25, and 32 days after implantation. The area of the disc infiltrated by fibroblasts was measured by planimetry. After 32 days exposure to hypoxic conditions (7 days before sponge implantation and 25 days after) EGF slightly increased (NS) the area of fibroblast infiltration compared to placebo under both hypoxic and normoxic conditions. No significant differences were observed between the hypoxically conditioned groups and normoxic controls. Neither chronic hypoxia alone nor chronic hypoxia with intermittent hyperbaric oxygen administered 21-32 days after disc implantation affected the area of fibroblast infiltration. EGF significantly increased the area of the fibrous capsule around small PVA sponges after 15 days under normoxic conditions. Fibroblast-Hyperbaric Oxygen-Hypoxia-Polyvinyl Alcohol Sponge Wound.