Detection of Francisella tularensis in Blood by Polymerase Chain Reaction.(Reannouncement with New Availability Information)

摘要

We developed a polymerase chain reaction-based assay for Francisella tularensiswhich we evaluated by using spiked blood samples and experimentally infected mice. The assay detected both type A and type B F. tularensis at levels equivalent to one CFU microliters of spiked blood. Results from polymerase chain reaction-based assay of limiting dilutions of blood from mice infected with the live vaccine strain agreed closely with results from blood culture.