Characteristics of the Biochemical Detector Sensor

摘要

The biosensor module of the Biochemical Detector (BCD) concept model is composeof two major subsystems, a light-addressable potentiometric (LAP) sensor and a fluidic processor. The biosensor module is automated to allow for uptake, mixing, and incubation of reagents and sample, filtration of the incubated mixture of reagents and sample through a nitrocellulose membrane, and detection of the membrane-immobilized analyte complexes by means of the LAP sensor. This report describes a series of experiments that were undertaken to compare the performance of the concept model with that of a commercial LAP sensor unit and an earlier breadboard version of the biosensor module of the BCS. Although the performance of the Lap sensor itself was essentially the same in all three units, it was found that for immunoassays which required mixing and incubation of reagents and sample, the output signals of the LAP sensor were generally lower on the concept model and breadboard than on the commercial unit. The differences in response are attributed to insufficient mixing of reagents and sample in the fluidic processors.