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What can we learn about cell signalling by combining optical imaging and patch clamp techniques?

机译:通过结合光学成像和膜片钳技术,我们可以了解有关细胞信号传导的哪些知识?

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Optical imaging is a powerful technique with which to investigate the activity, distribution and movement of biomolecules. The increased resolution of images obtained with confocal microscopy now allows us to visualize the signalling events in individual intracellular organelles. Local photobleaching and uncaging of caged compounds enable investigators to control the activity of many biologically important molecules in small localized regions of both cytosol and internal spaces of cellular organelles. Uncaging and photobleaching conveniently complement laser scanning confocal microscopy. The whole-cell recording configuration of the patch-clamp technique has been widely used not only to measure ionic currents, but also to control the concentration of important molecules in the cytosol. The cell-attached configuration of patch clamp was utilized for local stimulation of the cell and local delivery of the second messengers. This paper describes the advantages of combining patch-clamp and optical imaging methods as well as some of the recent achievements using this approach.
机译:光学成像是一项强大的技术,可用于研究生物分子的活性,分布和运动。通过共聚焦显微镜获得的图像分辨率的提高现在使我们能够可视化单个细胞内细胞器中的信号传导事件。笼状化合物的局部光漂白和解笼使研究者能够控制细胞溶质和细胞器内部空间的局部区域中许多重要生物学分子的活性。开箱和光漂白方便地补充了激光扫描共聚焦显微镜。膜片钳技术的全细胞记录配置已被广泛用于不仅测量离子电流,而且还用于控制细胞质中重要分子的浓度。膜片钳的细胞附着结构用于细胞的局部刺激和第二信使的局部递送。本文介绍了将膜片钳和光学成像方法相结合的优势,以及使用这种方法的最新成果。

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