Molecular markers in potato cultivars treated with ribosome-inactivating proteins. (Special Issue: Potato pathology.)

摘要

Most plants produce ribosome-inactivating proteins (RIPs) that inhibit protein synthesis through modification of RNA. In this report we studied the inheritance of resistance to Potato virus Y (PVY) in five potato cultivars treated with RIPs using ISSR-PCR since genetic information on this topic is limited. Leaf extracts from pokeweed (Phytolacca americana and P. acinosa) and the marvel of Peru (Mirabilis jalapa) were sprayed on five potato cultivars ('Selan', 'Spunta', 'Cara', 'Diamond' and 'Nicola') before virus inoculation, inhibiting infection by almost 100%, corroborated by DAS-ELISA. Changes in DNA (relative to PVYNTN-infected plants and the healthy control) in potato cultivars pretreated with 100 micro g/ml AVP resulted in genetic variations detected by ISSR-PCR analysis, which was performed using five primers. A total of 63 scorable amplified DNA fragments ranging from 9 0 to 1105 bp were observed using these primers, 43 of which were polymorphic while the others were monomorphic. The five primers showed a mean polymorphic percentage of 68.25%, the highest percentage derived from primer ISSR-1 (87.50%). The AVP-pretreated potato cultivars varied considerably in banding patterns using the five ISSR-PCR primers. 'Nicola' had the highest number of unique markers (9), followed by 'Selan' (8), 'Spunta' (5), 'Dimond' (1) and 'Cara' (0). Leaf extracts from P. americana, P. acinosa and M. jalapa could be used in simple crop-protection agricultural systems by spraying these extracts on leaves of various crops to prevent or control viral infection.CAS Registry Numbers 9007-49-2