Fermentation medium optimization for the biosynthesis of protease by Penicillium chrysogenum in shake flasks

摘要

Present investigation describes the optimization of fermentation media parameters for improved production of protease by Penicillium chrysogenum IHH5 in shake flasks. Out of different agro-industrial byproducts used as substrates for increased production of enzyme, soybean meal was found to be the best substrate. Glucose and peptone proved to be the best carbon and nitrogen sources for the biosynthesis of protease by mould culture. Finally, a volume of 50ml of culture medium per 250 ml shake flask was found to be optimum in present studies.