Next-Generation Sequencing and CAGE: Introducing New Methods for Transcriptome Analysis

摘要

Next-generation sequencing approaches are rapidly changing the way we are looking at genome sequencing. However, they also offer a powerful solution for transcriptome analysis. Here we summarise our results obtained by Cap Analysis Gene Expression or CAGE. CAGE obtains short sequence tags from 5' ends of mRNAs for transcript and transcription start site identification, expression profiling, and genome annotation. Linking expression values to promoter information has already led to new insights on the utilisation of genomic information and will further drive transcriptional networks and marker discovery.