Whole-cell mediated Baeyer-Villiger oxidation of functionalized bicyclo[3.3.0]ketones by recombinant E-coli

摘要

Recombinant whole cells of Escherichia coli overexpressing Acinetobacter sp. NCIMB 9871 cyclohexatione monooxygenase (F.C. 1.14,13.22) have been utilized for the Baeyer-Villiger oxidation of functionalized bicyclo [3.3.0] ketones. Prochiral substrates were designed to probe the impact of polarity and spatial configuration of the functional groups on conversion and enantio-selectivity. The data give additional insight into the steric requirements of the active site of the enzyme for high optical purity. The synthetic utility of the engineered E. coli strain is demonstrated and contributions to the non-natural substrate profile available so far are provided. [References: 49]