Identification and quantitation of proteins using mass spectrometry-based peptide multiplex labelling methods

摘要

Mass spectrometry-based tagging meth-ods enable the relative expression levelsof large sets of proteins to be meas-ured and quantitated with a high degreeof automation. One of these technolo-gies uses isobaric tagging for the simul-taneous identification and quantificationof proteins from multiple samples in asingle multiplex analysis.' The isobaricpeptide tagging makes it possible topool protein samples without increasingthe complexity of the mass spectrometry(MS) analysis, or losing important post-translational modification (PTM) infor-mation. A new set of isobaric taggingreagents has recently become availablethat, for the first time, allows up to eightdifferent protein samples to be multi-plexed (8plex), doubling the numberof states that can be compared.' Thisprovides greater flexibility in the design ofexperiments, such as by allowing moreexperimental samples to be comparedwithin a single run or permitting inclusionof greater numbers of duplicates withinthe same sample for increased statisti-cal support. The 8plex approach also hasthe potential to increase sample through-put while reducing the costs of time andlabour.