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Isolation and partial characterization of starches from dry beans (Phaseolus vulgaris) and chickpeas (Cicer arietinum), grown in Sonora, Mexico

机译:墨西哥索诺拉州种植的干豆(菜豆)和鹰嘴豆(Cicer arietinum)中淀粉的分离和部分表征

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摘要

For a better understanding of the carbohydrate components of Mexican dry legumes, in an attempt to suggest alternative processing, starches from dry beans (Phaseolus vulgaris var. pinto) and chickpeas (Cicer arietinum var. surutato) were isolated through sedimentation procedure. They were further characterized in their: physical characteristics of shape and size by light and Scanning Electron Microscopy (SEM); chemical characteristics by proximate analyses and amylose contents; functional characteristics of gelatinization temperature, viscosity and thermal properties by Differential Scanning Calorimetry (DSC); and nutritional characteristics, % digestibility. Starch in these two legumes was firstly isolated at over 85% recovery, with an amylose content of 52.4% and 46.5% for dry beans and chickpeas, respectively. SEM and light microscopy showed starch granules with a predominantly oval shape and size in the range of 20-25 mu, for both legumes. Higher gelatinization temperatures were found for dry beans than for chickpeas. Similarly, fusion enthalpies and maximum gelatinization temperature were also higher for beans, as shown by DSC. However, chickpea starch gave a slightly higher % digestibility than bean starch. These results suggested that the difference in the amylose content, in both legumes, determine the starch granule structure, their intermolecular organization and their physical and functional characteristics.
机译:为了更好地理解墨西哥干豆科植物的碳水化合物成分,尝试提出其他加工方法,通过沉淀法分离了干豆(菜豆(Phaseolus vulgaris var。pinto))和鹰嘴豆(Cicer arietinum var.surutato)中的淀粉。它们的特征还在于:通过光和扫描电子显微镜(SEM)的形状和尺寸的物理特征;通过直接分析和直链淀粉含量测定化学特性;通过差示扫描量热法(DSC)测定糊化温度,粘度和热性能的功能特征;和营养特性,消化率%。首先分离出两种豆类中的淀粉,回收率超过85%,干豆和鹰嘴豆的直链淀粉含量分别为52.4%和46.5%。扫描电镜和光学显微镜显示两种豆类的淀粉颗粒主要为椭圆形,大小在20-25微米之间。发现干豆的糊化温度高于鹰嘴豆。同样,如DSC所示,豆的融合焓和最高糊化温度也更高。但是,鹰嘴豆淀粉的消化率略高于豆淀粉。这些结果表明,两种豆类中直链淀粉含量的差异决定了淀粉颗粒的结构,分子间的组织以及它们的物理和功能特性。

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