首页> 外文期刊>Stem Cells >Single Adult Kidney Stem/Progenitor Cells Reconstitute Three-Dimensional Nephron Structures In Vitro
【24h】

Single Adult Kidney Stem/Progenitor Cells Reconstitute Three-Dimensional Nephron Structures In Vitro

机译:单个成年肾干/祖细胞在体外重建三维肾单位结构。

获取原文
获取原文并翻译 | 示例
           

摘要

The kidneys are formed during development from two distinct primordial tissues, the metanephric mesenchyme and the ureteric bud. The metanephric mesenchyme develops into the kidney nephron, the minimal functional unit of the kidney. A nephron consists of several segments and regulates water, electrolyte, and acid-base homeostasis in addition to secreting certain hormones. It has been predicted that the kidney will be among the last organs successfully regenerated in vitro due to its complex structure and multiple functions. Here, we show that adult kidney stem/progenitor cells (KS cells), derived from the S3 segment of adult rat kidney nephrons, can reconstitute a three-dimensional kidney-like structure in vitro. Kidney-like structures were formed when a cluster of KS cells was suspended in an extracellular matrix gel and cultured in the presence of several growth factors. Morphological analyses revealed that these kidney-like structures contained every substructure of the kidney, including glomeruli, proximal tubules, the loop of Henle, distal tubules, and collecting ducts, but no vasculature. Our results demonstrate that a cluster of tissue stem/progenitor cells has the ability to reconstitute the minimum unit of its organ of origin by differentiating into specialized cells in the correct location. This process differs from embryonic kidney development, which requires the mutual induction of two different populations of progenitors, metanephric mesenchymal cells and ureteric bud cells.
机译:肾脏是在发育过程中由两个不同的原始组织组成的,即后肾间质和输尿管芽。后肾间充质发展为肾肾单位,即肾的最小功能单位。肾单位由几个部分组成,除分泌某些激素外,还调节水,电解质和酸碱稳态。据预测,由于其复杂的结构和多种功能,肾脏将是在体外成功再生的最后器官之一。在这里,我们显示,成年大鼠肾肾的S3片段衍生的成年肾干/祖细胞(KS细胞)可以在体外重建三维肾样结构。当将KS细胞簇悬浮在细胞外基质凝胶中并在几种生长因子存在下培养时,形成肾脏样结构。形态学分析显示,这些肾样结构包含肾脏的每个子结构,包括肾小球,近端小管,亨利环,远端小管和收集管,但没有脉管系统。我们的结果表明,一组组织干/祖细胞具有通过在正确的位置分化成专门的细胞来重构其起源器官的最小单位的能力。此过程不同于胚胎肾脏发育,后者需要两个不同的祖细胞群,即后肾间充质细胞和输尿管芽细胞相互诱导。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号