Biosynthetic Incorporation of Fluorohistidine into Proteins in E.coli:A New Probe of Macromolecular Structure

摘要

Histidine is important for carrying out a number of protein functions.For instance,it can act as a general acid/base in enzymatic catalysis,it plays a role in ligating metal cations in metalloproteins,and stabilizes protein structure by metal binding,hydrogen bonding,or electrostatic interactions.One noteworthy example of histidine's potential role in protein stability is observed in the pathogenesis of the anthrax toxin,where protonation of His side chains in one of the proteins leads to a large structural perturbation and subsequent toxicity.