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Vector comprising antisense gene which partially inhibits acetic acid metabolism in E.coli and a process for preparation of recombinant proteins from E.coli transformed with the said vector
Vector comprising antisense gene which partially inhibits acetic acid metabolism in E.coli and a process for preparation of recombinant proteins from E.coli transformed with the said vector
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机译:包含部分抑制大肠杆菌中乙酸代谢的反义基因的载体和由所述载体转化的大肠杆菌制备重组蛋白的方法
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摘要
The present invention, the present invention more specifically relates to a recombinant protein production method of the E. coli transformed with vector and the electric vector containing the antisense gene to suppress parts of acetic acid metabolic pathway of E. coli, the two involved in the acetate generated enzyme acid phosphatase a (acetate kinase a, ackA) and phospho-trans-acetyl la azepin vector which part inhibited in antisense (antisense) express the mRNA levels of acid metabolic enzyme through gene expression (phosphotransacetylase, pta) and using the same. recombinant proteins related to production methods.; According to the invention, to resolve the recombinant protein production decreased and the cell growth inhibition problems caused by the second excessive discharge of the metabolite, acid when culturing an E. coli for recombinant protein production in a high concentration on the type of recombinant protein, unlike the conventional method It can be expressed in a high yield, regardless of which is it possible to solve the problem of high cost due to the process approach. In addition, it is expected that the economic impact of producing large quantities of expensive protein at a low production cost is greater by solving the cell growth inhibition and protein productivity reduction due to acetic acid emissions from glucose medium.
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