Fertility of Drosophila melanogaster Females Affected by Mutation l(2)M167~(DTS)

摘要

We studied the fertility of D. melanogaster females heterozygous for the dominant temperature sensitive mutation l(2)M167~(DTS), which exerts a recessive lethal effect at 25°C, under the conditions of stable temperature regimes 25, 28, and 29°C andchanging regimes 25 - 29°C and 29 - 25°C. It was shown that inhibition of total activity of oogenesis due to a decreased number of functioning ovarioles is one of the mechanisms underlying the decreased fertility of l(2)M167~(DTS)/+ females. Analysis of individual fertility of each female confirmed also the role of sterility as a component of fertility of the females. Sterilization was realized due both to full depletion of functioning ovarioles and disturbed mechanism of laying the mature eggs onto asubstrate as a result of violation of the feedback blocking normal ovulation, which led to the breakdown of ovarioles and filling of the abdominal cavity with mature oocytes. A significant polymorphism of heterozygous females by their fertility was observed. The intensity of sterilization and mortality of l(2)M167~(DTS)/+ females sharply increased at an elevated temperature (29°C), especially at the pupal stage.