ANTIOXIDANT AND ANTIGLYCATION POTENTIAL OF POLYPHENOL EXTRACTS OF SELECTED VEGETABLES FROM PAKISTAN

摘要

Current piece of research work was designed to focus on the antioxidant and antiglycation properties of five selected vegetables viz. Ipomoea batatas, Trigonella foenum-graecum, Daucus carota, Solanum melongena and Brassica rapa rapa those are locally available and readily consumed. In this context, optimisation of solvent extraction (methanolic) for the isolation of bioactive molecules was carried out. The biological assays bared a considerable antioxidant potential of selected green materials. Among vegetables, Trigonella foenum-graecum was proved to be superior while Brassica rapa rapa was considered to be inferior for phenolics and flavonoids content. Trigonella foenum-graecum also presented significant antioxidant activity with maximum reduction capacity and also having least IC50 as a result of inhibition of free radical scavenging by DPPH method. Early and intermediary glycation products through NBT reduction and Girard T tests revealed significant inhibitory effects of Trigonella foenum-graecurn and Solanum melongena at different incubation periods. The selected vegetables also exhibited a considerable inhibition for glucose-induced advanced glycation end-product (AGE) in a dose and time dependent manner in in vitro bovine serum albumin (BSA)-glucose system. As oxidation and glycation are relevant to diabetic complications the result of current work revealed that plant derived foods enriched with phytoprotectants are effective to tailor specific healthy diet for the target population.