High-Performance Liquid Chromatographic Analysis of Anthraquinone Compounds in the Laurera benguelensis

摘要

A high-performance liquid chromatographic (HPLC) method has been developed for the characterization of anthraquinone metabolites in extracts of the lichen Laurera benguelensis. With this method four anthraquinone derivatives 1,8-dihydroxy-3-methoxy-6-methylanthraquinone, 1,8-dihydroxy-3-formy1-6- methoxyanthraquinone, 1,8-dihydroxy-3-hydroxymethy1-6-methoxy-anthraquinone and 1,3, 8-trihyroxy6-methylanthraquinone can be analyzed. Components of lichen were detected by characteristic ultraviolet spectra and relative retention times. This is first report of phytochemical analysis of L. benguelensis. Importance of this research is in recognizing some new source (lichen and its extracts) as a natural emplacement of antioxidants because oxidation with free radicals or autooxidation is big problem for preservation of food products.