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Specifics of the coat protein gene in Russian strains of the cucumber green mottle mosaic virus

机译:俄罗斯黄瓜绿斑驳花叶病毒株外壳蛋白基因的细节

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摘要

The primary structure of the coat protein (CP) gene was examined for pathogenic strain MS-1 and vaccine strain VIROG-43M of the cucumber green mottle mosaic virus (CGMMV). In CP amino acid composition, strains MS-1 and VIROG-43M are typical representatives of CGMMV: their CPs have 98–100% homology to CPs of other tobamoviruses of the group. The CP gene has the same nucleotide composition in pathogenic MS-1 and vaccine VIROG-43M, indicating that strain attenuation is not determined by this gene. The CP amino acid sequences of the two Russian strains are fully identical to the CP sequences of two Greek strains, GR-3 and GR-5. However, the nucleotide sequences of their genes differ in 13 bp, testifying to the difference between the Russian and Greek strains.
机译:检查了黄瓜绿斑驳花叶病毒(CGMMV)的致病性菌株MS-1和疫苗菌株VIROG-43M的外壳蛋白(CP)基因的一级结构。在CP氨基酸组成中,菌株MS-1和VIROG-43M是CGMMV的典型代表:它们的CP与该组其他烟草花叶病毒的CP具有98-100%的同源性。 CP基因在致病性MS-1和疫苗VIROG-43M中具有相同的核苷酸组成,这表明该基因不能确定菌株的减毒作用。两个俄罗斯菌株的CP氨基酸序列与两个希腊菌株GR-3和GR-5的CP序列完全相同。但是,它们基因的核苷酸序列相差13 bp,这证明了俄罗斯和希腊菌株之间的差异。

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