Anthocyanins Identification, Separation and Measurement from Cranberry, Blueberry, Bilberry, Chokeberry and Acai Berry Extracts by HPTLC

摘要

In this paper is presented a high performance quantitative method for the quantification of anthocyanins (mv 3-glc, cy 3-glc, pn 3-glc and dp 3-glc) from cranberry, blueberry, bilberry, chokeberry and acai berry extracts. The anthocyanins were extracted using acidified methanol (4:1) and separated on HPTLC 60 F-254 silica gel plates with ethyl acetate, 2-butanone, water and formic acid 7/3/0.8/1.2 (v / v / v / v) as mobile phase within 30 min. The plates evaluation was made in white light transmitted after development. The correlation coefficient of the calibration curves was >= 0.99988 and the analysis repeatability was calculated on three applications of the same analyzed sample (<= 3.6%). Based on the results obtained, this method can be used in routine analysis for the anthocyanins determination from the above extracts. In order to identify the unknown compounds, mass spectrometry was used. The bioactivity and antioxidant properties of anthocyanins were highlighted by the Aliivibrio fisheri bacteria and DPPH * detection.