Lack of evidence for association between DVWA gene polymorphisms and developmental dysplasia of the hip in Chinese Han population.

摘要

Developmental dysplasia of the hip is the most frequent inborn deformity of the locomotor apparatus. Genetic factors play a considerable role in pathogenesis of Developmental dysplasia of the hip. Recently, several DVWA SNPs were found to be consistent and most significantly associated in Japanese and Han Chinese knee OA studies. Its functions may be associated with cartilage. It may be involved in etiology and pathogenesis of Developmental dysplasia of the hip. Our objective is to evaluate whether the DVWA SNPs (rs7639618, rs9864422 and rs11718863) are associated with Developmental dysplasia of the hip in Han Chinese. Three SNPs rs7639618, rs9864422 and rs11718863 (in DVWA) were genotyped using a Taqman 5' allelic discrimination assay on an ABI 7500 real-time polymerase chain reaction (PCR) instrument in 368 children who suffered from Developmental dysplasia of the hip and 508 control subjects, and analyzed their associations. The genotype distribution and allele frequency were compared between Developmental dysplasia of the hip and healthy control. Neither genotype distributions nor allelic frequencies of the assayed single nucleotide polymorphisms were found to be significantly different between patients and controls. There was also no significant difference when the patients were stratified by sex or severity (all P > 0.05). Our results indicate that DVWA does not seem to be a risk factor for Developmental dysplasia of the hip etiology in Chinese Han population.