Somaclonal variation of rice calli-clones regenerated after different periods of callus culture

摘要

Variations arisen from cell culture are called somaclonal variation (Larkin and Scowcroft 1981). We have been investigating how somaclonal variations occur and what kinds of variations are produced by the cell culture. Seeds of Oryza sativa L cv. Akihikari were used to induce calli and to culture during different periods. Two plant populations regenerated from four (short) and seven (long) months old calli were named as Aki-C and Aki-E, respectively. Regenerated plants were defined as RO generation,Next generation after self fertilization from RO plants was called as R1 in this experiment. Although control population generated from seeds without any culture did not show any variations, several kinds of induced variations were classified as, 1) albino, 2) xantha, stripe, and alboviridis, 3) dwarf, 4) variations for seed fertility, and 5) variations for days to heading.