Effect of ethanol, methanol, zinc, manganese and boron seed priming on ageing, seed germination and physiological characteristics in canola under water deficit stress

摘要

For studying the effects of seed priming with ethanol, methanol, Zn, B and Mn on seed mineral concentration and water deficit stress tolerance in canola (Brassica napus L.), two separate laborptory and greenhouse experiments were conducted. Laboratoryexperiment was performed as a ompletely randomized design with six priming treatments : Non-primed, 2% ethanol, 2% methanol and 0.5% each ZnSo_4, H_3B0_3 and MnS0_4.H_20 solutions in three replicates. Greenhouse experiment was conducted as a factorial experiment form by completely randomized design with four levels of irrigation : 100, 75, 50 and 25% FC with three replications. The analysis of variance showed significant effect of seed priming on the final germination percentage (GP), mean germinationtime (MGT), mean daily germination (MDG), daily germination speed (DGS) and germination rate index (GI). Furthermore, the analysis of variance showed a significant difference of seed priming and water deficit stress on the number of seeds per pod and 100-grain weight. The detailed results of the study showed that priming seeds with ethanol increased GP by 15%, MGT by 43%, MDG by 15% and GI by 67% over the untreated control. Besides, the results also proved that ethanol seed priming increased the numberof seeds per pod and 100-grain weight.