Phytochemical Screening and Antimicrobial Activity of Anthraquinones Isolated from Different Parts of Cassia nodosa

摘要

The study aims in investigating the phytochemical screening and antimicrobial activity of isolated anthraquinones from different parts of Cassia nodosa against various bacterial and fungal strains to confirm their effective use in different traditional medicines against these pathogenic strains. Fresh plant parts of C. nodosa were collected, dried at room temperature, powdered and extraction of anthraquinones were done using standard protocol. Extracted compounds were screened by UV, IR, ~1HNMR, ~(13)CNMR techniques. Agar well diffusion method was used to test the antimicrobial activity of isolated anthraquinones against pathogenic microbial strains. The phytochemical screening revealed the presence of Emodin and Rhein in the plant. Maximum level of Emodin (0.66 mg gdw~(-1)) and Rhein (0.30 mg gdw~(-1)) was observed in leaves. The crude compounds exhibited a significant antimicrobial activity. The emodin demonstrated maximum activity against F. moniliforme (IZ = 26.00 mm) while rhein was more active against A. flavus (IZ = 21.00 mm). The inhibitory effects of Emodin and Rhein extracted from various parts of C. nodosa against various pathogenic micro-organisms had clearly demonstrated the usefulness of C. nodosa in the treatment of various diseases caused by these pathogenic strains.