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首页> 外文期刊>Reproductive biomedicine online >Effect of antioxidant supplementation of cryopreservation medium on post-thaw integrity of human spermatozoa.
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Effect of antioxidant supplementation of cryopreservation medium on post-thaw integrity of human spermatozoa.

机译:抗冻剂补充抗氧化剂对人精子融化后完整性的影响。

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摘要

This study examined whether the addition of an antioxidant to cryopreservation medium could improve the post-thaw integrity of cryopreserved human spermatozoa, particularly from men with abnormal semen parameters. Semen samples were collected by masturbation and assessed following WHO standards. Normal (n = 23) and abnormal (n = 20) samples were divided into three aliquots prior to cryopreservation. The first aliquot remained untreated and was mixed with cryopreservation medium (in-house 1:1). The second and third aliquots were mixed with cryopreservation medium containing either 100 micromol or 200 micromol vitamin E analogue. Samples were frozen at -10 degrees C per minute to -80 degrees C, then plunged into liquid nitrogen. Thawed samples were assessed for motility, vitality and DNA integrity. Split-plot repeated-measures ANOVA was used to assess within-subject (dose) and between-group (normal/abnormal) differences in post-thaw motility index, vitality staining and DNA fragmentation. Vitamin E dose was significantly associated with post-thaw motility (P = 0.041) and the pattern of response across doses was similar for normal and abnormal groups. Post-thaw motility was significantly improved by the addition of 200 micromol vitamin E (P = 0.006), but neither vitality nor sperm DNA fragmentation were altered. These results suggest that the addition of vitamin E to cryopreservation medium improves post-thaw motility.
机译:这项研究检查了在冷冻保存培养基中添加抗氧化剂是否可以改善冷冻保存的人类精子的融解后完整性,尤其是精液参数异常的男性。通过手淫收集精液样本,并根据WHO标准进行评估。在冷冻保存之前,将正常(n = 23)和异常(n = 20)样品分成三等份。第一份等分试样未经处理,并与冷冻保存培养基(内部1:1)混合。将第二和第三等分试样与包含100微摩尔或200微摩尔维生素E类似物的冷冻保存介质混合。将样品以每分钟-10摄氏度的速度冷冻至-80摄氏度,然后放入液氮中。评估解冻后样品的活力,活力和DNA完整性。分裂图重复测量方差分析用于评估解冻后运动指数,活力染色和DNA片段化的受试者内(剂量)和组间(正常/异常)差异。维生素E剂量与解冻后的运动能力显着相关(P = 0.041),并且正常和异常组的不同剂量反应模式相似。添加200微摩尔维生素E可显着改善解冻后的运动能力(P = 0.006),但活力和精子DNA片段均未改变。这些结果表明,向冷冻保存培养基中添加维生素E可改善解冻后的运动能力。

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