黄芪多糖对人精子冷冻后DNA完整性的保护作用

摘要

目的：研究黄芪多糖（AP）对冻融人精子DNA完整性的保护作用。方法：收集正常（7份）和低活力（5份）共12例患者的精液标本，分别用含0，10，25，50 mg/L AP和1 g/L维生素E（VE）的冷冻保护液冻存精液，经过常规冻融和吖啶橙染色后，在荧光显微镜下计算精子的DNA碎片指数（DFI）。结果：在正常精子组中，T1（含10 mg/L AP）样本的DFI值比T0（含0 mg/L AP）、T2（含25 mg/L AP）、T3（含50 mg/L AP）和T4（含1 g/L VE）样本降低（P＜0.05）；在低活力精子组中，T1样本的DFI值低于T0和T4样本，差异有统计学意义（P＜0.05）。结论：AP在适当浓度时（10 mg/L）对冷冻保存的人精子DNA完整性有保护作用。%Objective:To examine the protective effect of astragalus polyose (AP) on the DNA integrity of human sperm underwent freezing-thawing. Methods:The human semen samples with normal motility (7 samples) or low motility (5 samples) were collected. After grouped, the sperm suspension were freezed with the cryoprotective medium contained 0, 10, 25 and 50 mg/L of AP (the T0, T1, T2, T3 groups). The positive control is 1 g/L of Vitamin E (the T4 group). After the conventionally freezing-thawing, and dyeing with acridine orange, those sperm samples were detected the DNA fragmentation index (DFI) using fluorescence microscope. Results:In those groups of normal sperm motility, the DFI in the T1 group was significantly lower than those in the T0, T2, T3 and T4 groups (P<0.05). In those groups of low sperm motility, the DFI in the T1 group was significantly lower than those in the T0 group and the T4 group (P<0.05). Conclusions:The AP at 10 mg/L concentration has a protective effect on the DNA integrity of human sperm underwent freezing-thawing.