Influences of lipopolysaccharide-induced immune challenge on performance and whole-body protein turnover in weanling pigs

摘要

The objective of this study is to characterize the influence of immune stress induced by lipopolysaccharide (LPS) on protein utilization and turnover for early-weaned pigs. A total of 15, crossbred weanling pigs (initial body weight 10.15 +/- 0.39 kg) were assigned to one of three treatments. Pigs were injected with LPS and fed ad libitum (LPS-challenge), or injected with endotoxin-free physiological salt solution (PSS) and fed ad libitum (control), or injected with PSS and fed the same amount of feed as LPS-challenged pigs (pair-feed). All pigs received a 4-d nitrogen balance trial. On d I and 3 of the trial, LPS-challenged pigs were injected intramuscularly with 200 mu g/kg BW of LPS dissolved in 1 ml PSS. Pigs in other treatments were injected with 1 ml PSS. N-15-Glycine (5 mg/kg BW) was gastrically infused after the second injection. Feces and urine were collected daily to determine the N output throughout the duration of the trial. Lymphocyte blastogenesis (LB) and serum immunoglobulins (IgA, IgG, IgM) were also detected to illustrate the LPS-induced immune responses. Results indicated that the injection of LPS significantly (P<0.01) elevated the LB and resulted in lower average daily gain (ADG), feed intake (ADFI) and efficiency of feed utilization than control pigs. Pair-fed pigs had higher performance than LPS-challenged pig but poorer than control pigs. Injection of LPS also resulted in significantly (P<0.01) lower nitrogen intake and efficiency of utilization than controls, and more fecal N excretion than Pair-fed pigs (3.19 +/- 0.85 vs. 2.19 +/- 0.67 g/d, P<0.05). The whole-body nitrogen flux (4.34 +/- 0.19 vs. 11.35 +/- 0.12 g N/kg BW0.75/d) and N accretion (5.57 +/- 0.59 vs. 10.17 +/- 1.12 g Pr/kg BW0.75/d) were acutely (P<0.01) reduced as the feed intake decreased, but there was no significant difference between LPS-challenged and pair-fed pigs. Injection of LPS markedly (P<0.05) increased the protein degradation (16.76 +/- 1.09 vs. 14.53 +/- 1.24 g N/kg BW0.75/d). It is concluded that LPS-induced immune challenge depresses growth performance and feed utilization efficiency by enhancing protein degradation rate and decreasing protein utilization for body protein retention. (C) 2007 Published by Elsevier B.V.