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A transgenic porcine clonal somatic cell line expressing human FasL protein to inhibit cellular immunity and transgenic clone pig using the same
A transgenic porcine clonal somatic cell line expressing human FasL protein to inhibit cellular immunity and transgenic clone pig using the same
PURPOSE: A transgenic porcine clonal somatic cell line which expresses human FasL gene in a tissue and cells is provided to reduce cytotoxicity and to minimize immune rejection. CONSTITUTION: A transgenic porcine clonal cell line contains human FasL gene expression vector and is deposited in the number KCLRF-BP-00194. A method for preparing the somatic cell line comprises: a step of isolating somatic cells from porcine fetus; a step of preparing hFasL gene expression vector and introducing to the somatic cells; and a step of selecting expression vector-introduced somatic cells and culturing.
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