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A smartphone-based chip-scale microscope using ambient illumination

机译:使用环境照明的基于智能手机的芯片级显微镜

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摘要

Portable chip-scale microscopy devices can potentially address various imaging needs in mobile healthcare and environmental monitoring. Here, we demonstrate the adaptation of a smartphone's camera to function as a compact lensless microscope. Unlike other chip-scale microscopy schemes, this method uses ambient illumination as its light source and does not require the incorporation of a dedicated light source. The method is based on the shadow imaging technique where the sample is placed on the surface of the image sensor, which captures direct shadow images under illumination. To improve the image resolution beyond the pixel size, we perform pixel super-resolution reconstruction with multiple images at different angles of illumination, which are captured while the user is manually tilting the device around any ambient light source, such as the sun or a lamp. The lensless imaging scheme allows for sub-micron resolution imaging over an ultra-wide field-of-view (FOV). Image acquisition and reconstruction are performed on the device using a custom-built Android application, constructing a stand-alone imaging device for field applications. We discuss the construction of the device using a commercial smartphone and demonstrate the imaging capabilities of our system.
机译:便携式芯片级显微镜设备可以潜在地满足移动医疗和环境监控中的各种成像需求。在这里,我们演示了智能手机相机如何适应紧凑型无透镜显微镜的功能。与其他芯片级显微镜方案不同,此方法使用环境照明作为其光源,并且不需要并入专用光源。该方法基于阴影成像技术,其中将样本放置在图像传感器的表面上,该图像传感器在照明下捕获直接的阴影图像。为了将图像分辨率提高到超出像素大小的水平,我们使用不同照明角度的多个图像执行像素超分辨率重建,这些图像是在用户手动将设备围绕任何环境光源(例如太阳或灯)倾斜时捕获的。无透镜成像方案允许在超宽视野(FOV)上进行亚微米分辨率成像。使用定制的Android应用程序在设备上执行图像采集和重建,从而为现场应用程序构建独立的成像设备。我们将讨论使用商用智能手机的设备结构,并演示我们系统的成像功能。

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