A novel affinity chromatography method for the co-purification of deoxycytidine kinase and cytidine deaminase.

Uchida H; cbio.acbio.fukui-u.ac.jp; Morinaga H; Misaki T; Miyazaki T; Uwajima T; Obata T; Endo Y; Matsuda A; Sasaki T

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A novel affinity chromatography method for the co-purification of deoxycytidine kinase and cytidine deaminase.

机译：一种用于共纯化脱氧胞苷激酶和胞苷脱氨酶的新型亲和层析方法。

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摘要

By affinity chromatography with Sepharose coupled to 2'-deoxy-1-beta-D-ribofuranosyl-N4-dodecanoylcytosine, deoxycytidine kinase and cytidine deaminase were purified 1,950- and 2,240-fold, respectively, from Ehrlich carcinoma cells, and their enzyme activities for several deoxycytidine analogs were investigated.

机译：通过琼脂糖凝胶与2'-脱氧-1-β-D-核呋喃糖基-N4-十二烷酰胞嘧啶偶联的亲和色谱法，分别从Ehrlich癌细胞中纯化出1,950-和2,240-倍的脱氧胞苷激酶和胞苷脱氨酶，以及它们的酶活性研究了几种脱氧胞苷类似物。

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《Nucleosides, nucleotides and nucleic acids》 |2001年第9期|共8页
作者
Uchida H; cbio.acbio.fukui-u.ac.jp; Morinaga H; Misaki T; Miyazaki T; Uwajima T; Obata T; Endo Y; Matsuda A; Sasaki T;
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正文语种 eng
中图分类细胞形态学;
关键词
Chromatography; Affinity; Cytidine Deaminase; Deoxycytidine Kinase; 色谱法; 亲和; 胞苷脱氨酶; 脱氧胞苷激酶;

机译：Chromatography;Affinity;Cytidine Deaminase;Deoxycytidine Kinase;色谱法;亲和;胞苷脱氨酶;脱氧胞苷激酶;

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1. A novel affinity chromatography method for the co-purification of deoxycytidine kinase and cytidine deaminase. [J] . Uchida H, cbio.acbio.fukui-u.ac.jp, Morinaga H, Nucleosides, nucleotides and nucleic acids . 2001,第9期

机译：一种用于共纯化脱氧胞苷激酶和胞苷脱氨酶的新型亲和层析方法。
2. A comparison of the enantioselectivities of human deoxycytidine kinase and human cytidine deaminase. [J] . Shafiee M, Griffon JF, Gosselin G, Biochemical Pharmacology . 1998,第9期

机译：人脱氧胞苷激酶和人胞嘧啶脱氨酶的对映选择性的比较。
3. Isolation of deoxycytidine kinase from Ehrlich carcinoma cells by affinity chromatography based on a substrate analog, 2'-C-cyano-2'-deoxy-1-beta-D-arabinofuranosyl-N4-palmitoylcytosine ++ +. [J] . Uchida H, Morinaga H, Hayashi Y, Biological & pharmaceutical bulletin . 1999,第1期

机译：基于底物类似物2'-C-氰基-2'-脱氧-1-β-D-阿拉伯呋喃糖基-N4-棕榈酰胞嘧啶++ +的亲和色谱法从埃希氏癌细胞中分离脱氧胞苷激酶。
4. Gas-Phase Conformations, Energetics, and Mechanisms for Glycosidic Bond Dissociation of Protonated 2'-Deoxycytidine and Cytidine [C] . Ranran Wu, Mary T. Rodgers ASMS Conference on Mass Spectrometry and Allied Topics . 2015

机译：气相构象，能量学和质子化2'-脱氧胞苷和胞苷的糖苷键解离的机制
5. I) The Development of Deoxycytidine Kinase Inhibitors with Nanomolar Affinity and Improved Metabolic Stability. II) The Synthesis of Ferrocene-Containing Monomers and Biodegradable Polymers Using Azide-Alkyne Click Chemistry and Ring Opening Polymerization [D] . Gipson, Raymond Marshall 2015

机译：I）具有纳摩尔摩尔亲和力和改善的代谢稳定性的脱氧胞苷激酶抑制剂的开发。 II）使用叠氮化物-炔烃Click化学和开环聚合反应合成含二茂铁的单体和可生物降解的聚合物
6. Paclitaxel alters the expression and specific activity of deoxycytidine kinase and cytidine deaminase in non-small cell lung cancer cell lines [O] . Stacy S Shord, Shitalben R Patel 2009

机译：紫杉醇改变非小细胞肺癌细胞系中脱氧胞苷激酶和胞苷脱氨酶的表达和比活性
7. Efficient deamination of 5-methylcytidine and 5-substituted cytidine residues in DNA by human APOBEC3A cytidine deaminase. [O] . Rodolphe Suspène, Marie-Ming Aynaud, Jean-Pierre Vartanian, 2013

机译：通过人apOBEC3a胞苷脱氨酶对DNa中的5-甲基胞苷和5-取代的胞苷残基进行有效脱氨基。

A novel affinity chromatography method for the co-purification of deoxycytidine kinase and cytidine deaminase.

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