首页> 外文期刊>Neuroscience: An International Journal under the Editorial Direction of IBRO >Nerve injury induces the expression of EXT2, a glycosyltransferase required for heparan sulfate synthesis.
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Nerve injury induces the expression of EXT2, a glycosyltransferase required for heparan sulfate synthesis.

机译:神经损伤诱导了EXT2的表达,EXT2是硫酸乙酰肝素合成所需的糖基转移酶。

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摘要

Heparan sulfate proteoglycans, which bear long chains of heparan sulfate glycosaminoglycan, play significant roles during embryogenesis, including the formation of the CNS. However, their involvement in nerve regeneration has not yet been clarified. Here, we found that the mRNA expression of EXT2, one of the crucial enzymes for heparan sulfate-glycosaminoglycan synthesis, was markedly up-regulated in injured hypoglossal motor neurons after axotomy. In addition, immunohistochemical staining with an antibody specific for heparan sulfate-glycosaminoglycan chains demonstrated increased expression of heparan sulfate-glycosaminoglycan chains in the injured nucleus. Furthermore, the mRNA expressions of glypican-1 and syndecan-1, which are both well-known heparan sulfate proteoglycans, were prominently up-regulated in injured motor neurons. These results suggest that the biosynthesis of heparan sulfate chains promoted by EXT2 is activated in injured motor neurons, and that glypican-1 and syndecan-1 are potent candidates for heparan sulfate proteoglycans involved in peripheral nerve regeneration.
机译:带有硫酸乙酰肝素糖胺聚糖长链的硫酸乙酰肝素蛋白聚糖在胚胎发生包括CNS形成过程中起重要作用。但是,它们在神经再生中的作用还不清楚。在这里,我们发现轴索切开后受损的舌下运动神经元中EXT2(硫酸乙酰肝素-糖胺聚糖合成的关键酶之一)的mRNA表达明显上调。另外,用对硫酸乙酰肝素-糖胺聚糖链具有特异性的抗体的免疫组织化学染色证明了在受损细胞核中硫酸乙酰肝素-糖胺聚糖链的表达增加。此外,众所周知的硫酸乙酰肝素蛋白聚糖glypican-1和syndecan-1的mRNA表达在受伤的运动神经元中明显上调。这些结果表明,EXT2促进的硫酸乙酰肝素链的生物合成在受伤的运动神经元中被激活,而glypican-1和syndecan-1是参与周围神经再生的硫酸乙酰肝素蛋白聚糖的有效候选者。

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